phytochemical screening and evaluation of antioxidant activities of dracocephalum kotschyi and determination of its luteolin content

نویسندگان

mansureh kamali department of chemical engineering, quchan azad university, quchan, iran

susan khosroyar department of chemical engineering, quchan azad university, quchan, iran

hossein kamali natural products & medicinal plants research center, north khorasan university of medical sciences, bojnurd, iran

tooba ahmadzadeh sani school of pharmacy, mashhad university of medical sciences, mashhad, iran

چکیده

objective: dracocephalum kotschyi (lamiaceae family) has been usedin traditional medicine for stomach and liver disorders, headache and congestion. in the present study, we have investigated phytochemical properties and antioxidant activities of dichloromethane, ethyl acetate and methanol extracts of d.kotschyi. material and methods: antioxidant activities of extracts were evaluated using the integration of hplc-dpph and ferric reducing antioxidant power (frap) methods. in addition, the luteolincontent was determined using hplc method. results: the highest antioxidant activity was observed for the methanol extract (among the three tested extracts) showing 50% dpph scavenging activity at 4.85µg/ml as compared to butylated hydroxy toluene (bht) and ascorbic acid (3.00 µg/ml, 0.97 µg/ml). also, luteolin was detected in methanol extract; it was identified by comparing its retention time and dad spectra with standard and it was one of antioxidant components of this plant. in addition, the antioxidant activity of methanol extract was higher than bht, in frap assay. total phenolic content was in the range of 11.62-22.29 mg gallic acid /gram of dry extract and flavonoid content was in the range of 3.97-5.042 mg             quercetin/ gram of extract for dichloromethane, ethyl acetate and methanol extracts. the quantity of luteolin in d.kotschyiwas found to be 1061.005 µg/g of dried plant. conclusion: the results of this investigation indicated that luteolin plays major role in the antioxidant activity of the plant.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Phytochemical screening and evaluation of antioxidant activities of Dracocephalum kotschyi and determination of its luteolin content

Objective: Dracocephalum kotschyi (Lamiaceae family) has been usedin traditional medicine for stomach and liver disorders, headache and congestion. In the present study, we have investigated phytochemical properties and antioxidant activities of dichloromethane, ethyl acetate and methanol extracts of D.kotschyi. Material and Methods: Antioxidant activities of extracts were evaluated using the i...

متن کامل

Phytochemical screening and evaluation of antioxidant activities of Dracocephalum kotschyi and determination of its luteolin content

OBJECTIVE Dracocephalum kotschyi (Lamiaceae family) has been used in traditional medicine for stomach and liver disorders, headache and congestion. In the present study, we have investigated phytochemical properties and antioxidant activities of dichloromethane, ethyl acetate and methanol extracts of D.kotschyi. MATERIAL AND METHODS Antioxidant activities of extracts were evaluated using the ...

متن کامل

the study of practical and theoretical foundation of credit risk and its coverage

پس از بررسی هر کدام از فاکتورهای نوع صنعت, نوع ضمانت نامه, نرخ بهره , نرخ تورم, ریسک اعتباری کشورها, کارمزد, ریکاوری, gdp, پوشش و وثیقه بر ریسک اعتباری صندوق ضمانت صادرات ایران مشخص گردید که همه فاکتورها به استثنای ریسک اعتباری کشورها و کارمزد بقیه فاکتورها رابطه معناداری با ریسک اعتباری دارند در ضمن نرخ بهره , نرخ تورم, ریکاوری, و نوع صنعت و ریسک کشورها اثر عکس روی ریسک اعتباری داردو پوشش, وثی...

15 صفحه اول

the innovation of a statistical model to estimate dependable rainfall (dr) and develop it for determination and classification of drought and wet years of iran

آب حاصل از بارش منبع تأمین نیازهای بی شمار جانداران به ویژه انسان است و هرگونه کاهش در کم و کیف آن مستقیماً حیات موجودات زنده را تحت تأثیر منفی قرار می دهد. نوسان سال به سال بارش از ویژگی های اساسی و بسیار مهم بارش های سالانه ایران محسوب می شود که آثار زیان بار آن در تمام عرصه های اقتصادی، اجتماعی و حتی سیاسی- امنیتی به نحوی منعکس می شود. چون میزان آب ناشی از بارش یکی از مولفه های اصلی برنامه ...

15 صفحه اول

Phytochemical Screening, Antioxidant and Antibacterial Activities of Commiphora kerstingii

Phytochemical screening, antioxidant and antibacterial activities of aqueous and methanolic crude extracts of Commiphora kerstingii leaves were investigated. The antioxidant and antibacterial properties of different solvent extracts of Commiphora kerstingii plant were scrutinized. The phytochemical screening of the various extracts was carried out using standard methods and the results revealed...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
avicenna journal of phytomedicine

جلد ۶، شماره ۴، صفحات ۴۲۵-۴۳۳

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023